Journal: Journal of Advanced Research

Article Title: Synergistic potentiation of the anti-metastatic effect of a Ginseng - Salvia miltiorrhiza herbal pair and its biological ingredients via the suppression of CD62E-dependent neutrophil infiltration and NETformation

doi: 10.1016/j.jare.2024.10.036

Figure Lengend Snippet: Rg1 reduced NETs in lung tissues. (A) Representative immunofluorescence images and quantification of H3cit and NE in the lungs (n = 3; scale bar: 50 μm). (B) Representative western blot images of H3cit and NE levels in the lungs and quantitative data ( n = 3). GAPDH was used as a loading control. (C-E) Serum levels of CXCL1, CXCL2, and G-CSF were detected via ELISA ( n = 10). (F-H) The mRNA expression levels of CXCL1, CXCL2 and G-CSF in lung tissues were determined by qRT–PCR ( n = 3). All the data are presented as mean ± SEM. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. the model group.

Article Snippet: The levels of mouse CXCL1, CXCL2, and G-CDF in the serum were measured via the Mouse CXCL1/KC ELISA Kit (EK296/2, Multi Sciences), Mouse CXCL2/MIP-2 ELISA Kit (EK2142, Multi Sciences), and Mouse G-CSF ELISA Kit (EK269/2, Multi Sciences) according to the manufacturers’ instructions.

Techniques: Immunofluorescence, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR